Wu Lab Website

Doctoral Thesis Title: The mechanism and function of pervasive noncoding transcription in the mammalian genome

Publications while at MIT: 

1. Cell-Type-Specific Alternative Splicing Governs Cell Fate in the Developing Cerebral Cortex.

Zhang X, Chen MH, Wu X, Kodani A, Fan J, Doan R, Ozawa M, Ma J, Yoshida N, Reiter JF, Black DL, Kharchenko PV, Sharp PA, Walsh CA.

Cell. 2016 Aug 25;166(5):1147-1162.e15. doi: 10.1016/j.cell.2016.07.025.PMID:27565344 Free PMC Article

2.  In vivo genome editing using Staphylococcus aureus Cas9.

Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F.

Nature. 2015 Apr 9;520(7546):186-91. doi: 10.1038/nature14299. Epub 2015 Apr 1. PMID:25830891 Free PMC Article

 3. Target specificity of the CRISPR-Cas9 system.

Wu X, Kriz AJ, Sharp PA.

Quant Biol. 2014 Jun;2(2):59-70. PMID: 25722925 Free PMC Article

4.  Global microRNA depletion suppresses tumor angiogenesis.

Chen S, Xue Y, Wu X, Le C, Bhutkar A, Bell EL, Zhang F, Langer R, Sharp PA.

Genes Dev. 2014 May 15;28(10):1054-67. doi: 10.1101/gad.239681.114. Epub 2014 May 1. PMID: 24788094 Free PMC Article

 5. Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells.

Wu X, Scott DA, Kriz AJ, Chiu AC, Hsu PD, Dadon DB, Cheng AW, Trevino AE, Konermann S, Chen S, Jaenisch R, Zhang F, Sharp PA.

Nat Biotechnol. 2014 Jul;32(7):670-6. doi: 10.1038/nbt.2889. Epub 2014 Apr 20.PMID:24752079 Free PMC Article

6.  Divergent transcription: a driving force for new gene origination?

Wu X, Sharp PA.

Cell. 2013 Nov 21;155(5):990-6. doi: 10.1016/j.cell.2013.10.048.PMID:24267885 Free PMC Article

7. Single-molecule mRNA detection and counting in mammalian tissue.

Lyubimova A, Itzkovitz S, Junker JP, Fan ZP, Wu X, van Oudenaarden A. Nat Protoc. 2013 Sep;8(9):1743-58. doi: 10.1038/nprot.2013.109. Epub 2013 Aug 15.PMID:23949380

8. Promoter directionality is controlled by U1 snRNP and polyadenylation signals.

Almada AE, Wu X, Kriz AJ, Burge CB, Sharp PA.

Nature. 2013 Jul 18;499(7458):360-3. doi: 10.1038/nature12349. Epub 2013 Jun 23. PMID: 23792564 Free PMC Article